Nonmicellar systems for solution NMR spectroscopy of membrane proteins
نویسندگان
چکیده
منابع مشابه
Structure of outer membrane protein G by solution NMR spectroscopy.
The bacterial outer membrane protein G (OmpG), a monomeric pH-gated porin, was overexpressed in Escherichia coli and refolded in beta-octyl glucoside micelles. After transfer into dodecylphosphocholine micelles, the solution structure of OmpG was determined by solution NMR spectroscopy at pH 6.3. Complete backbone assignments were obtained for 234 of 280 residues based on CA, CB, and CO connect...
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ژورنال
عنوان ژورنال: Current Opinion in Structural Biology
سال: 2010
ISSN: 0959-440X
DOI: 10.1016/j.sbi.2010.05.006